Coding

Part:BBa_K3595011:Design

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)


Mutant cysE-256-5-11-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CGT (Arg) to CAT (His) 265-267 ACC (Thr) to GTA (Val) 268-270 CCG (Pro) to GCT (Ala) 277-279 GCA (Ala) to ACA (Thr) 280-282 GTC (Val) to AGA (Arg) 283-285 GAT (Asp) to CCC (Pro) 286-288 ATG (Met) to TGG (Trp) 766-768



Source

Synthetic

References